14.4 Folate (categories)

Folate range (<45.3 vs >=45.3)

14.4.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 231.6615844 1 <0.0001 ***
Folate_gp 3.4750812 1 0.06 ns
Study 0.1842697 1 0.7 ns
GA_samp 173.4460939 1 <0.0001 ***
Folate_gp:Study 0.9042110 1 0.3 ns
hPL (μg/ml)
Chisq Df Pr(>Chisq)
(Intercept) 43.4448740 1 <0.0001 ***
Folate_gp 0.0364404 1 0.8 ns
Study 15.0574283 1 0.0001 ***
GA_samp 2495.8520938 1 <0.0001 ***
Folate_gp:Study 0.2603451 1 0.6 ns
GH2 (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 426.8274799 1 <0.0001 ***
Folate_gp 0.0062344 1 0.9 ns
Study 0.0027234 1 1.0 ns
GA_samp 1070.4519710 1 <0.0001 ***
Folate_gp:Study 1.4933308 1 0.2 ns
Insulin (uU/ml)
model term df1 df2 F.ratio Chisq p.value
1 Folate_gp 1 Inf 3.067 3.067 0.08 ns
3 Study 1 Inf 11.682 11.682 0.0006 ***
4 GA_samp 1 Inf 0.913 0.913 0.3 ns
2 Folate_gp:Study 1 Inf 1.258 1.258 0.3 ns
HOMA-IR
model term df1 df2 F.ratio Chisq p.value
1 Folate_gp 1 Inf 3.018 3.018 0.08 ns
3 Study 1 Inf 25.541 25.541 <0.0001 ***
4 GA_samp 1 Inf 0.445 0.445 0.5 ns
2 Folate_gp:Study 1 Inf 1.185 1.185 0.3 ns
Glucose (mmol/L)
model term df1 df2 F.ratio Chisq p.value
1 Folate_gp 1 Inf 0.159 0.159 0.7 ns
3 Study 1 Inf 111.887 111.887 <0.0001 ***
4 GA_samp 1 Inf 0.556 0.556 0.5 ns
2 Folate_gp:Study 1 Inf 0.203 0.203 0.7 ns

14.4.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)
Var Effect P
(Intercept) 15.51 (10.89, 22.08) <0.0001
Folate_gp≥45.3 0.94 (0.88, 1.00) 0.06
StudySTOP 0.99 (0.89, 1.10) 0.85
GA_samp 1.15 (1.13, 1.18) <0.0001
hPL (μg/ml)
Var Effect P
(Intercept) 1.86 (1.55, 2.23) <0.0001
Folate_gp≥45.3 0.99 (0.96, 1.03) 0.72
StudySTOP 1.12 (1.06, 1.18) 0.0001
GA_samp 1.34 (1.32, 1.36) <0.0001
GH2 (ng/ml)
Var Effect P
(Intercept) 0.07 (0.06, 0.10) <0.0001
Folate_gp≥45.3 0.96 (0.92, 1.01) 0.13
StudySTOP 0.99 (0.92, 1.07) 0.78
GA_samp 1.30 (1.28, 1.32) <0.0001
Insulin (uU/ml)
Var Effect P
(Intercept) 12.69 (8.04, 20.00) <0.0001
Folate_gp≥45.3 0.90 (0.82, 0.99) 0.03
StudySTOP 0.80 (0.69, 0.92) 0.002
GA_samp 1.01 (0.99, 1.04) 0.34
HOMA-IR
Var Effect P
(Intercept) 3.24 (2.00, 5.24) <0.0001
Folate_gp≥45.3 0.90 (0.81, 0.99) 0.03
StudySTOP 0.69 (0.59, 0.80) <0.0001
GA_samp 1.01 (0.98, 1.04) 0.50
Glucose (mmol/L)
Var Effect P
(Intercept) 5.68 (5.19, 6.20) <0.0001
Folate_gp≥45.3 1.00 (0.98, 1.02) 0.73
StudySTOP 0.86 (0.84, 0.88) <0.0001
GA_samp 1.00 (0.99, 1.00) 0.45

14.4.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

PRL (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 96.00630 7.195117 86.43610 82.71826 111.4290
STOP 95.07406 6.813547 72.37696 82.41817 109.6733
hPL (μg/ml)
Study response SE df lower.CL upper.CL
SCOPE 88.26468 2.363507 155.2526 83.71723 93.05914
STOP 98.64043 2.427366 110.9325 93.94580 103.56966
GH2 (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 2.307938 0.0656473 440.8614 2.182457 2.440633
STOP 2.283240 0.0546164 255.4347 2.178178 2.393369
Insulin (uU/ml)
Study response SE df asymp.LCL asymp.UCL
SCOPE 14.52554 0.7204407 Inf 13.17996 16.00848
STOP 11.56468 0.4431398 Inf 10.72796 12.46667
HOMA-IR
Study response SE df asymp.LCL asymp.UCL
SCOPE 3.525094 0.1852528 Inf 3.180079 3.907541
STOP 2.417761 0.0982142 Inf 2.232728 2.618128
Glucose (mmol/L)
Study response SE df asymp.LCL asymp.UCL
SCOPE 5.490652 0.0518795 Inf 5.389906 5.593281
STOP 4.724282 0.0359866 Inf 4.654274 4.795344

14.4.4 Comparisons (Overall)

Hormone Contrast Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) ≥45.3 / <45.3 0.94 (0.88, 1.00) 0.06
hPL (μg/ml) ≥45.3 / <45.3 0.99 (0.96, 1.03) 0.7
GH2 (ng/ml) ≥45.3 / <45.3 0.96 (0.92, 1.01) 0.1
Insulin (uU/ml) ≥45.3 / <45.3 0.90 (0.82, 0.99) 0.03
HOMA-IR ≥45.3 / <45.3 0.90 (0.81, 0.99) 0.03
Glucose (mmol/L) ≥45.3 / <45.3 1.00 (0.98, 1.02) 0.7

14.4.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

14.4.6 Plots